المساهمة في دراسة العلاقة الكمية والنوعية للمحتوى الفينولي ودراسة النشاطية المضادة للأكسدة لعســل ونبات المرخ

Abstract

The objective of this work was to study the quantitative and qualitative relationship of polyphenolic and antioxidant activity of the methanolic extract of the aerial part, flowers and the honey of Genista saharae Coss and Dur. (Almarakh) produced in the region of Oued Souf. In order to achieve the study, we first evaluated the yield of the methanolic extract for each vegetative part of the phase flowering and flowers, the results showed that the latter was superior by 11.72%. For quantitative estimation of total phenols, the aerial part gave the largest amount followed by flowers and then honey and there values were respectively (1.034±0.055 mg E AG / g Ext), (0.471 ± 0.245 mg E AG / g Ext),) 0.71±0.09 mg E AG / 100g honey). Secondly, we evaluated the antioxidant activity of these samples using free radical’s DPPH. , These extracts showed a weak activity compared to ascorbic acid and recorded the lowest inhibitory values of the root at honey; As for the test of the dissolution of red blood cells (hemolysis). Results have demonstrated the ability of plant extracts and honey licensed to protect red blood cells from dissolution by free radicals, and the percentages were as follows: 84% for the part of the phase flowering, and 39% of the flowers (at concentration of 1.5 mg / ml) and honey at 48% (at 0.8 g / l concentration). Thirdly, we analyzed the phenolic compounds by HPLC, of which the following compounds were known: gallic acid (1.032 μg / mg Ext in the part of the phase flowering only) and ρ- Coumarin (65.670 μg / mg Ext in flowers, 31.60 μg / mg Ext in the aerial part) and (0. 377 μg / mg Ext in honey). Rutin (8.813 μg / mg Ext at flowers and 3.398 μg / mg Ext in the aerial part). In order to obtain a qualitative characterization according to the international standards of honey used in this study, we studied some of the physiochemical criteria, including: humidity, density, pH, ash, electrical conductivity, refractive index and sugar content, we found it within the international standards and standards of high quality.