PHENOL BIODEGRADATION AND OPTIMI S ATION OF CULTURAL AND PHYSICAL CONDITIONS BY THE FREE CELLS OF NEWLY ISOLATED PHENOL DEGRADING ALCALIGENES SP. AQ5 02

Abstract

B iological t reatment is understood to be the most effi cient technique for phenol removal paralleled to other physio chemical methods. 16s rRNA sequencing for the identification . Mineral salt media with 0.5 g/L phenol as the only carbon source. Temperature pH, salinit y and nitrogen source were optimi s ed . The effects of heavy metals on the percentage of phenol degradation with were tested A ccession number of KT693288 was assigned after identification . Temperature of 25 35° C pH 7 8 phosphate buffer were the optimum a nd a mmonium sul ph ate was established to be the paramount nitrogen source at 0.4 0.5 g/L for isolate. The optimi s ed conditions were found reducing the incubation period to 48 h with the ability to tolerate up to 0.2 g/L sodium chloride and degraded 50% an d 1.1 g/L phenol. Meanwhile, the isolate AQ 5 02 can also effectively degrade 1000 g/L phenol in the presence of heavy metals such as Cr, Zn, and Fe .